ASA 10-3, Issue No. 138, June 25, 2010

Prof. Elena Ryabchikova and her colleagues are the first in the world to show the details of cell interactions and nanoparticles. Their results raise very serious questions as to the fundamental science, as well as to the responsibilities of the developers of drugs, food supplements, cosmetics and other products that use titanium oxide and other nanomaterials. Many researchers and companies are looking to create nanoforms of different materials for a variety of applications from health care products to energy production and distribution, to industrial chemical production and to drug delivery systems. Is it reasonable to use nanoparticles before understanding their interactions with cells? Will nanoparticles damage cells or will they be a useful carrier for the delivery of drugs into cells? The importance of this research, as well as answers to many questions, can not be overstated.

Structural Features Of TiO2 Nanoparticles Interaction With A Cell

Elena I. Ryabchikova1, Natalia A. Mazurkova2, Nadejda V. Shikina3, Zinfer R. Ismagilov3,
Stanislav N. Zagrebel'niy2

 1Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Science, Lavrent'eva pr. 8, Novosibirsk, 630090 Russia

2Novosibirsk State University, Pirogova str. 2, Novosibirsk, 630090 Russia

3Institute of Catalysis, Siberian Branch of Russian Academy of Science. Lavrent'eva pr.5, Novosibirsk, 630090 Russia


We examined the interaction of amorphous, anatase, brookite and rutile TiO2 nanoparticles (4—5 nm) with MDCK cells. The cells were incubated in presence of 100 µg/mL TiO2 nanoparticles during 1, 3 and 5 h, and then fixed for electron microscopy. The number of living MDCK cells was counted, using trypan blue assay, after 5 and 15 h treatment with 100 µg/mL TiO2 nanoparticles at 37oC. All of the TiO2 nanoparticles were toxic to the MDCK cells. Different modes of cell-nanoparticle interaction were observed with the four types of TiO2. The interaction of amorphous, anatase and brookite TiO2 nanoparticles involve intimate cellular mechanisms. This calls for careful examination of nanoparticle-cellular interaction before introducing such particles into living tissues or the environment.

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Dr. Benjamin Garrett, with this historical profile on Dr. Gabriel Bertrand, continues in his very well conceived and received series on our fellow professionals that have influenced the direction and course of history across the convoluted arena of chemical warfare.

Profiles in CW History:
Gabriel Bertrand &
The Bertrand No. 1

Benjamin Garrett Ph.D.

French biological chemist Gabriel Émile Bertrand (1867-1962) is noted for his pioneering studies on enzymes. He is credited as the first person to suggest that oxidizing enzymes contain metals. He is also credited with suggesting the terms oxidase for an oxidizing enzyme (1894) and co-enzyme for a generally low molecular weight organic substance that attaches itself to a protein to form an active enzyme (1897). In the context of chemical warfare, Bertrand performed various services immediately prior to and throughout World War I for the French government.

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an inside look at ASA 10-3

The Professional Library
Group picture — the CBMTS Eight Plenary
The CBMTS VIII Executive Summary


Participant pictures — CBMTS VIII


Focal Point News — Chris Dishovsky
Army CBRN Modernization
Solicitation: Rapid Collection of CBN Residue
Health Notes of Interest to CBRN professionals
Likelihood of Smallpox Recurrence - a look 23
Current Contracts

For the Professional in Government and Industry with an interest in Nuclear, Biological and Chemical Defense, Disarmament and Verification; Emergency and Disaster Medical Planning; Industrial Health and Safety; and Environmental Protection

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